96 Well Plasmid Kit
Model Number IB47153
The 96 well IBI plasmid kit was designed for high-throughput purification of plasmid DNA from cultured bacterial cells. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Shop online now or call for a quote!
- Item Description
- Specifications
- Protocol
- Other Plasmid Kits
Additional Item Description:
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or a vacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.
Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.
Key Details:
Specifications Chart:
| Specifications | 96-Well Plasmid Kit | Component | 2 x 96 Well | |
| Format: | 96-Well Plate | PD1 Buffer1: | 40 ml | |
| Binding Capacity: | 35 µg | PD2 Buffer2: | 40 ml | |
| Culture Input: | 1-5 ml | PD3 Buffer: | 60 ml | |
| Culture Type: | Bacteria culture | W1 Buffer: | 80 ml | |
| Plasmid Size: | 1-15 kb | Wash Buffer: | 25 ml | |
| Typical Yield: | 20-35 µg/ well | Elution Buffer: | 30 ml | |
| Elution Volume: | 100 µl | RNase A: (50 mg/ml) | 130 µl | |
| Operation Time: | 25 minutes | Plasmid 96 Well Filter Plate: | 2 (IB47150) | |
| Plasmid 96 Well Binding Plate: | 2 | |||
| 96 Deep Well Plate: | 2 (IB47153) | |||
| 0.35 ml Collection Plate: | 2 | |||
| Adhesive Film: | 8 |
Protocol Link:
Check out other Plasmid Kits by IBI:
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.
3. I-Blue Midi Plasmid Kit (Endotoxin-Free)
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
4. Maxi Fast Ion Plasmid Kit (Endotoxin-Free)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
6. I-Blue Mini Plasmid Kit (Endotoxin-Free)
The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The High Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures.
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