
Agarose for PFGE – High-Strength Agarose for Electrophoresis & Molecular Biology
IBI PFGE Agarose is a high-performance, molecular biology-grade agarose designed for pulsed field gel electrophoresis (PFGE), standard gel electrophoresis, blotting, and biomolecule immobilization. This ultra-pure agarose gel provides exceptional gel strength, high thermal stability, and superior resolution, making it ideal for separating large DNA fragments exceeding 1,000 base pairs. Its low sulfate content reduces electroendosmosis (EEO), ensuring sharper bands and more efficient separation of nucleic acids.
High-Strength Agarose for PFGE and DNA Separation
The strong intercatenary interactions within this high-resolution agarose create a robust gel matrix with a high exclusion limit, preventing gel breakage and distortion during electrophoresis. Designed for precise separation of genomic DNA, chromosomal analysis, and bacterial typing, IBI PFGE Agarose is also DNase- and RNase-free, making it an excellent choice for sensitive molecular biology workflows.
In addition to PFGE applications, this molecular biology agarose is optimized for standard agarose gel electrophoresis, Southern and Northern blotting, and enzyme or cell immobilization. Its low absorption of staining agents ensures clear visualization with ethidium bromide, SYBR dyes, and other nucleic acid stains. The advanced formulation provides consistent gel performance, high reproducibility, and contamination-free results, making it the preferred choice for researchers in genomics, microbiology, and diagnostic laboratories.
IBI Agarose for PFGE offers unmatched clarity, durability, and resolution, ensuring optimal performance for high-precision electrophoresis and molecular biology experiments.
CAS#: 9012-36-6
Product Video:
- Physical Specifications
- Certificate of Analysis
- SDS
Technical Specifications:
CAS#:9012-36-6
Moisture:≤7%
Ash:≤0.25%
EEO:≤0.12
Sulfate:≤0.12%
Clarity @ 1.5% (NTU):≤4
Gel Strength @ 1.0% (g/cm2):≥1800
Gel Strength @ 1.5% (g/cm2):≥3200
Gelling Temperature @ 1.5%:36°C ± 1.5°C
Melting Temperature @ 1.5%:88°C ± 1.5°C
Molecular Biology Specifications:
DNase assay:None Detected
RNase assay:None Detected
Protease assay:None Detected
Gel analysis assay:Pass
DNA Resolution:≥ 1000bp (Finely Resolved)
DNA Extraction assay:Pass
Gel Background:Very Low
Certificate of Analysis:
P.F.G.E. Agarose CofA - Lot# 14L4001
P.F.G.E. Agarose CofA - Lot# 15G3001
P.F.G.E. Agarose CofA - Lot# 16F3005
P.F.G.E. Agarose CofA - Lot# 17C5001
P.F.G.E. Agarose CofA - Lot# 18E3005
P.F.G.E. Agarose CofA- Lot#19D2014
P.F.G.E. Agarose CofA__Lot#21B2002