The IBI Scientific Isolate 96-well Total RNA Purification kit is based on a phenol and guanidine isothiocyanate solution coupled with a 96-well binding plate system for high throughput purification of high-quality Total RNA from a variety of samples. This system provides maximum Total RNA extraction from difficult samples high in polysaccharides and lipids. The binding plate and wash procedures ensure high purity of eluted RNA.
- Instructions
- Additional Item Description
- Specifications
- Protocol
- Parts & Accessories
How To Use:
To begin, samples are homogenized in IBI Scientific Isolate reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization simply bind, wash and elute the high-quality Total RNA in RNase Free water and use in a variety of sensitive downstream applications. Process is completed in as little as 60 minutes.
Additional Key Details:
- 2 x 96 well Isolate RNA kit, 4 x 96 well Isolate RNA kit or 10 x 96 well Isolate RNA kit.
- Vacuum or Centrifuge format
- Sample: Up to 2 x 10 6 cultured cells, 10 – 20 mg of animal tissue, up to 150 ul of body fluids, up to 1 x 10 9 bacteria cells, 10 – 20 mg of plant tissue.
- Binding Plates, Collection Plates and Deep well plates included.
- DNAse 1 reagent kit and Bacteria Lysis reagent kit (Lysozyme) sold separately.
IB47635 – 2 x 96-Well Total RNA Pure Kit
IB47636 - 4 x 96-Well Total RNA Pure Kit
IB47637 - 10 x 96-Well Total RNA Pure Kit
Applications: Tissue, Blood, Adherent cultured cells, Suspension cultured cells, Plant tissue, Bacteria and other body fluids such as buffy coat, serum and plasma