Large DNA Fragment Extraction Kits
Model Number IB47070
Efficient Recovery of Large DNA Fragments
The Large DNA Fragment Extraction Kit is meticulously engineered to recover or concentrate DNA fragments ranging from 100bp to 50Kb from agarose gel, PCR products, or enzymatic reactions. Using a concentrated sodium buffer, the kit effectively dissolves agarose gel and denatures enzymes, enabling DNA fragments to bind to silica beads. Contaminants such as salts, enzymes, and unincorporated nucleotides are removed using an ethanol-based Wash Buffer, with purified DNA eluted in low-salt Elution Buffer, TE Buffer, or water. This streamlined process eliminates the need for phenol extraction or alcohol precipitation, providing high-concentration DNA (10-20µl elution volume) ready for applications like cloning, sequencing, and enzymatic modifications—all within just 20-30 minutes.
Reliable and Easy-to-Use Performance
The kit features a built-in pH indicator for precise pH optimization and includes sodium acetate for pH adjustments after gel dissociation or PCR reactions. Designed for convenience and reliability, the Large DNA Fragment Extraction Kit undergoes rigorous lot-to-lot quality testing, ensuring consistent performance in recovering DNA fragments. With an impressive yield of 70-95% for fragments 100bp-10Kb and 60-80% for fragments over 10Kb, researchers can rely on this kit for superior purification results. Free samples are available upon request, making it an accessible choice for efficient DNA fragment recovery in diverse molecular biology workflows.
- Specifications
- Protocol
- Technical Information
Physical Specifications:
Sample Size: up to 250mg of agarose gel / up to 100ul of PCR product.
Format: Modified Silica bead and Spin column
Operation:Centrifuge
Max Fragment size:100bp – 50kb
Expected yield:100bp to 10kb = 70 – 95%, >10kb = 60 – 80%
Elution volume:10 – 20ul
Operation time:20 minutes for PCR Clean-up and 30 minutes for gel extraction
Applications:PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, Ligation.
Protocol Link:
PCR Clean-up and Gel Extraction Graph
What is the competition’s comparable kit?QIAGEN QIAEX II Gel Extraction Kit
I have SDS in my sample; will this adversely affect my gel extraction recovery?A small amount of SDS will not adversely affect the gel extraction recovery.
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