Replacement DF Columns and Collection Tubes

Key Details:

• For use with leftover reagents from IBI PCR/Gel Extraction Kits or competitive products
• Sample Size: 300mg agarose gel/100µl PCR product
• Elution Volume: 20 – 50µl
• Binding Capacity: Up to 10µg

You must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

Specifications:

Sample Size: 300mg of agarose gel or 100µl of PCR product
Format: Spin column or vacuum manifold
Expected Yield: 90% for Gel extraction and 95% for PCR Clean-up
Elution Volume: 20 - 50µl
Operation Time: 20 minutes
Purified DNA is ready for use in Fluorescent or Radioactive sequencing, ligation, PCR, restriction enzyme digestion, and DNA labeling.

Protocol:

Replacement PCR/Gel Extraction Columns Protocol