High-Speed Mini Plasmid Kit
Model Number IB47101
Efficient Plasmid DNA Extraction with High-Speed Mini Plasmid Kits
The High-Speed Mini Plasmid Kits by IBI Scientific are ideal for researchers needing quick and reliable plasmid DNA extraction from bacterial cultures. These kits utilize alkaline lysis and RNase treatment to isolate plasmid DNA while minimizing genomic DNA and RNA contamination. With the ability to process 1-5ml cultures, they are perfect for small to medium-scale plasmid extractions. The method ensures high-quality plasmid DNA, making it suitable for various downstream applications, including cloning, sequencing, and transfection experiments.
Fast and High-Yield Results for High and Low-Copy Plasmids
Designed for efficiency, the High-Speed Mini Plasmid Kits offer impressive yields in under 30 minutes. For high-copy plasmids, the kits can yield 20-30µg of DNA, while low-copy plasmids typically yield between 3-10µg. With a binding capacity of up to 30µg, the kits provide consistent, high-quality results. Whether you’re working with high-copy or low-copy plasmids, these kits are a fast, effective solution for obtaining pure plasmid DNA from bacterial cultures. Check out our basic guide for plasmid kits for more information.
- Specifications
- Protocol
- Technical Information
- Other Plasmid Kits
Physical Specifications:
| Specifications | High-Speed Mini Plasmid Kit |
| Format: | Spin Column |
| Binding Capacity: | 30 µg |
| Culture Input: | 1-5 ml |
| Culture Type: | Cultured bacterial cells |
| Plasmid Size: | 1-15 kb |
| Typical Yield: | 10-30 µg |
| Elution Volume: | 30-100 µl |
| Operation Time: | <15 minutes |
Protocol Link:
Technical Details:
In the presence of a chaotropic salt, the plasmid DNA in the lysate, binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol based wash buffer. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol plasmid. Typical yields are 20-30 µg for high-copy numbered plasmids or 3-10 µg for low-copy numbered plasmids. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. The entire procedure can be completed in under 30 minutes.
Quality Control:
The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis, by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid p Bluescript (A600>2U/ml). Following the purification process, a yield of more than 20 µg is expected and the ratio of A260/A280 is between 1.7-1.9. The purified plasmid (1 µg) is used in EcoRI digestion and checked by electrophoresis.
FAQs:
What competitor’s kit is comparable?
QIAGENS QIAprep Spin Miniprep Kit QIAGENS QuickLyse Miniprep KitCan QIAGEN'S reagents be used with IBI columns?
Yes, anything that is “lysis-based” will work.Is the use of ampicillin or other antibiotics during the cultivation of the E.coli necessary to achieve proper yield with the IBI mini plasmid kit?
Yes, in order to achieve good yield, the E.coli must be stressed with an antibiotic.How do I increase the Yield for this kit?
1. Increase your Lysis time 2. Pre-heat the Elution Buffer 3. Perform the Elution step twice 4. With a large/concentrated sample, end-user can add extra PD 1, 2, 3 buffersWhat’s the maximum amount of sample for the Mini Plasmid column/tube?
The maximum amount of sample for the column/tube is 4 ml.What is the primary make-up of the Elution buffer?
The primary make-up of the buffer is Tris-HCl pH 8.5. This buffer does not contain EDTA. You can also use Pure Water or TE buffer.Can the plasmid DNA extraction kit be applied on Gram (+) Bacteria?
Our plasmid kits are primarily designed to extract plasmid DNA from Gram (-) bacteria such as E. Coli. Gram (+) bacteria has thicker cell walls so the cell lysis buffers provided in the kit do not lyse them readily. However, extraction of plasmid DNA from Gram (+) bacteria can still be achieved with additional treatment. After re-suspending the pelleted bacterial cells in the PD1 Buffer, add lysozyme to give a final concentration of 3 to 5 mg/ml.What are the difference and/or purpose of the W1 Buffer vs. the Wash Buffer?
W1 contains chaotropic salt and ethanol. The chaotropic salt can inhibit and remove the enzyme activity, such as Taq polymerase and endonuclease. Wash Buffer contains low concentrations of salt and will remove salts and proteins from the final sample.Was the RNAse A volume increased in the Mini plasmid kits?
Yes, the amount of RNAse A was doubled on 9-23-14. The concentration remained the same – 50 mg/ml. The amount of RNAse added to the 25 ml bottle of P D1 is now 100 µl. The amount RNAse added to the 65 ml bottle of P D1 is 260 µl. This is double to what it was. This is for sequencing grade plasmid DNA.What’s the difference between the High-Speed Mini Plasmid Kit and the I-Blue Mini Plasmid Kits?
The differences are 2 X volume of RNAse A and I-Blue Lysis Buffer in the I-Blue Mini. The larger volume of RNAse A increases the yield for larger sample volumes. Hi Speed Mini allows for 1-5 ml and the I-Blue Mini allows for 1-7 ml which will yield more plasmid DNA. All other buffers are the same.Photo of Gel:
Photo 1- Comparision of Percent RecoveryConclusions:
1. IBI brand Purification Kits are equivalent or superior to Brand Q in Yield.
2. IBI brand Purification Kits are equivalent to Brand Q in Purity.
Check out other Plasmid Kits by IBI:
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.
3. I-Blue Midi Plasmid Kit (Endotoxin-Free)
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
4. Maxi Fast Ion Plasmid Kit (Endotoxin-Free)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
6. I-Blue Mini Plasmid Kit (Endotoxin-Free)
The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well.
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