I-Blue Mini Plasmid Kit (Endotoxin-Free)
Model Number IB47175
The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants.
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Additional Item Description:
In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified endotoxin free plasmid DNA is ready-for-use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions.
The quality of the I-Blue Mini Plasmid Endo-Free Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4ml overnightE. coli(DH5α) culture, containing plasmid pBluescript (A600>2U/ml). Following the purification process, a yield of more than 25µg is obtained and the ratio of A260/A280 is between 1.8-2.0. The purified plasmid DNA (1µg) is used in EcoRI digestion and analyzed by electrophoresis.
IBI offers the I-Blue Mini Plasmid Endo-Free Kit in 4 prep sample kits and 100 prep kits.
Key Details:
Specifications Chart:
| Specifications: | I-Blue Mini Plasmid Kit (Endotoxin-Free) |
| Format: | Spin Column |
| Binding Capacity: | 40 µg |
| Culture Input: | 1-5 ml |
| Culture Type: | Cultured E. coli cells |
| Plasmid Size: | 1-15 kb |
| Typical Yield: | up to 40 µg |
| Elution Volume: | 30-100 µl |
| Operation Time: | <60 minutes |
Protocol Link:
Shop our other Plasmid Kits below:
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.
3. I-Blue Midi Plasmid Kit (Endotoxin-Free)
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
4. Maxi Fast Ion Plasmid Kit (Endotoxin-Free)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
5. High-Speed Mini Plasmid Kit
The High-Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures.
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well.
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