Mini Total RNA Kit (Plants)
Model Number IB47340
Total RNA Mini Extraction Kit for Plants – High-Quality RNA Isolation in 30 Minutes
The Total RNA Mini Extraction Kit for Plants is a powerful and efficient solution for isolating high-purity total RNA from plant tissues and cells. This extraction kit is specifically designed to simplify RNA purification while ensuring maximum yield and integrity for molecular biology applications. Whether working with fresh plant tissue (up to 100 mg) or dry plant samples (up to 25 mg), researchers can expect RNA yields between 5–30 µg, depending on the sample type and quality.
Plant samples present unique challenges in RNA extraction due to their high levels of secondary metabolites and polysaccharides, which can interfere with downstream applications. This RNA extraction kit overcomes these obstacles with a high-efficiency spin column-based method that ensures superior RNA purity without the need for phenol extraction or alcohol precipitation. The entire extraction process can be completed in 30 minutes or less, allowing researchers to quickly obtain high-quality RNA for critical applications such as reverse transcription (RT), RT-PCR, Real-time PCR, and northern blotting.
Fast and Reliable RNA Extraction with High Purity and Yield
The Total RNA Mini Kit for Plants is designed to maximize RNA recovery while eliminating contaminants. The process starts by grinding plant samples in liquid nitrogen, ensuring complete cell lysis while preventing RNA degradation. The homogenized sample is then filtered to remove cell debris, leaving behind a clean lysate for the next steps.
A binding buffer containing chaotropic salts is then added to the lysate, promoting the selective binding of RNA molecules to the glass fiber matrix of the spin column. This critical step ensures efficient RNA capture while removing proteins, polysaccharides, and other impurities that may compromise RNA quality.
For experiments requiring pure RNA, an optional DNase treatment can be performed to remove any unwanted DNA contamination. Once the RNA is securely bound to the column, contaminants are washed away using a specialized ethanol-containing Wash Buffer, ensuring maximum sample purity. Finally, the purified RNA is eluted using RNase-free water, providing a clean, high-quality sample ready for immediate use in molecular biology applications.
Why Choose This RNA Extraction Kit?
This extraction kit is the ideal solution for researchers working with plant RNA, offering a fast, simple, and phenol-free purification process. Unlike traditional methods, which require time-consuming organic solvent extractions, this kit provides a safer and more efficient workflow with minimal hands-on time. The high-binding spin column technology ensures consistent and reproducible results, making it an indispensable tool for plant molecular biology research.
Each Total RNA Mini Extraction Kit for Plants undergoes rigorous quality control testing on a lot-to-lot basis. Total RNA is extracted from 25 mg of young leaf tissue, quantified using a spectrophotometer, and analyzed by electrophoresis to verify integrity. This ensures that researchers receive a high-performance kit capable of delivering reliable RNA quantification and excellent sample purity in every experiment.
The kit is specifically optimized for extracting RNA from a variety of plant tissues, including leaves, stems, roots, and flowers. Whether conducting gene expression studies, transcriptome analysis, or RNA sequencing, this extraction kit provides the precision and reliability needed for successful research.
Frequently Asked Questions (FAQs)
What is an RNA extraction kit?
An RNA extraction kit is a laboratory tool designed to isolate and purify RNA from biological samples. This process is crucial for studying gene expression, identifying genetic variations, and performing downstream molecular biology applications such as RT-PCR, sequencing, and northern blotting.
Why is this RNA extraction kit ideal for plant tissue?
Plant cells contain high levels of secondary metabolites, polysaccharides, and other contaminants that can interfere with RNA purification. The Total RNA Mini Extraction Kit for Plants is specifically designed to overcome these challenges by utilizing a specialized binding buffer and a high-efficiency spin column system, ensuring high-purity RNA recovery.
What makes this RNA extraction kit different from others?
This kit provides a phenol-free, fast, and efficient protocol that eliminates the need for organic solvents and time-consuming precipitation steps. The optimized buffer system and spin column technology allow for rapid purification while preserving RNA integrity, making it an excellent choice for plant molecular biology research.
Can this extraction kit be used for all plant types?
Yes, this kit is suitable for a wide range of plant species and tissue types, including young leaves, stems, flowers, and roots. However, RNA yield may vary depending on the plant species, tissue type, and developmental stage.
Does this RNA extraction kit remove DNA contamination?
Yes, the kit includes an optional DNase treatment step to eliminate any residual genomic DNA, ensuring high-purity RNA for applications such as RT-PCR and quantitative gene expression analysis.
Reliable RNA Extraction for Plant Molecular Biology Research
For researchers conducting plant molecular biology studies, having a reliable extraction kit is essential for obtaining high-quality RNA. The Total RNA Mini Extraction Kit for Plants provides an optimized, efficient, and contamination-free workflow, ensuring reproducible results in every experiment.
By using liquid nitrogen grinding, chaotropic salt-based binding, and spin column purification, this extraction kit guarantees maximum RNA yield and purity while minimizing degradation risks. With a fast 30-minute protocol and no hazardous organic solvents required, it is the ideal choice for plant RNA isolation in gene expression studies, transcriptome analysis, and RNA sequencing applications.
Upgrade your RNA extraction process with the Total RNA Mini Extraction Kit for Plants—ensuring superior RNA yield, purity, and consistency in every experiment.
- Specifications
- Protocol
- Total RNA Replacement Columns and Collection Tubes
- Replacement Buffers
Specifications Chart:
| Specifications | Mini Total RNA Kit (Plants) |
| Format: | Spin Column |
| Binding Capacity: | 50 µg |
| Sample Concentration: | 100 mg of fresh plant tissue 25 mg of dry plant tissue |
| Sample Type: | Plant tissue |
| Filter Column: | Yes |
| Typical Yield: | 5-30 µg |
| Elution Volume: | 50 µl |
| Operation Time: | <20 mins. |
Protocol Link:
Total RNA Columns:
Replacement Buffers Link:
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