rBAC Mini Total RNA Kit
Model Number IB47420
rBAC Mini Total RNA Extraction Kit – Fast, High-Purity RNA Isolation from Bacteria
The rBAC Mini Total RNA Extraction Kit is an advanced solution designed for the rapid and efficient purification of high-quality total RNA from gram-positive and gram-negative bacteria. This specialized extraction kit is optimized to break down bacterial cell walls, ensuring the complete isolation of RNA free from DNA contamination.
With a processing time of 30 minutes or less, this total RNA extraction kit provides researchers with a reliable and high-yield solution for isolating RNA from up to 1×10⁹ bacterial cells, delivering up to 60 µg of purified RNA per sample. The elution volume ranges from 50 to 100 µl, ensuring researchers can adjust RNA concentration based on experimental needs. Ideal for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, and cDNA Synthesis, this kit is a must-have for microbiology and molecular biology laboratories.
Efficient RNA Extraction for Gram-Positive and Gram-Negative Bacteria
One of the biggest challenges in bacterial RNA extraction is breaking through the complex cell walls of gram-positive and gram-negative bacteria. The rBAC Mini Total RNA Extraction Kit overcomes this by utilizing a gram-positive buffer that, when combined with lysozyme, efficiently digests bacterial cell walls consisting of peptidoglycan. This ensures complete lysis and high RNA recovery from even the most challenging bacterial strains.
Once cell walls are disrupted, chaotropic salts and detergents further lyse the bacterial cells while simultaneously inactivating RNases, preventing RNA degradation during the purification process. The resulting RNA-containing lysate is then applied to a high-efficiency spin column, where RNA selectively binds to the glass fiber matrix, leaving behind unwanted proteins and cellular contaminants.
To ensure maximum purity, an optional DNase treatment can be performed to eliminate any residual DNA, ensuring researchers obtain RNA of the highest integrity for downstream applications. Following a series of wash steps using an ethanol-based buffer, the purified RNA is finally eluted in RNase-free water, leaving behind a high-quality, contamination-free sample ready for immediate use.
Reliable and Consistent RNA Purification
This total RNA extraction kit is rigorously tested to guarantee consistent performance across batches. Each kit undergoes lot-to-lot quality control by extracting RNA from Escherichia coli (E. coli) cultures at a density of 1×10⁹ bacterial cells (OD600 = 1.3, 1 ml). The resulting RNA is analyzed by electrophoresis on a 0.8% agarose gel, ensuring that every kit meets the highest standards for purity, integrity, and yield.
Additionally, all IBI Total RNA columns are individually packaged and certified RNase- and DNase-free, ensuring that no external contamination interferes with sensitive RNA workflows.
Key Benefits of the rBAC Mini Total RNA Extraction Kit
The rBAC Mini Total RNA Extraction Kit provides a highly efficient and time-saving solution for researchers working with bacterial RNA. Unlike traditional extraction methods that involve time-consuming phenol-chloroform steps, this spin-column-based RNA extraction kit offers a safer and faster workflow, making it the preferred choice for high-throughput laboratories and research institutions.
Additionally, this kit’s flexible elution volume (50–100 µl) ensures that researchers can obtain highly concentrated RNA or dilute samples as needed, providing adaptability for a variety of downstream applications, including RNA sequencing, transcriptomics, and gene expression analysis.
Frequently Asked Questions (FAQs)
What is an RNA extraction kit?
An RNA extraction kit is a laboratory tool used to isolate and purify RNA from biological samples, such as bacterial cells. It ensures the removal of proteins, DNA, and other contaminants, allowing researchers to obtain high-quality RNA for use in molecular biology applications, such as RT-PCR and cDNA synthesis.
Why is an RNA extraction kit necessary for bacterial RNA isolation?
Bacteria, especially gram-positive strains, have complex cell walls that require specific buffers and enzymes for efficient RNA extraction. The rBAC Mini Total RNA Extraction Kit is designed to break down bacterial cell walls, inactivate RNases, and selectively bind RNA, ensuring high recovery and purity.
How does this extraction kit remove DNA contamination?
This kit includes an optional DNase treatment step, which effectively removes any residual genomic DNA, ensuring that the purified RNA is free from unwanted DNA contamination.
Can this RNA extraction kit be used for all bacterial species?
Yes, this kit is optimized for both gram-positive and gram-negative bacteria, making it versatile for use in various bacterial research applications. However, the lysozyme treatment step is particularly important for gram-positive bacteria, which have thicker cell walls composed of peptidoglycan.
What are the advantages of using this kit over traditional RNA extraction methods?
This extraction kit provides a safer and more efficient alternative to traditional phenol-chloroform extraction methods, which involve hazardous chemicals and lengthy protocols. With a quick 30-minute protocol, this kit simplifies the process while delivering high-yield RNA with excellent purity.
Can I get a free sample of this extraction kit?
Yes! Free samples of the rBAC Mini Total RNA Extraction Kit are available upon request. Contact us today to request a sample and evaluate the performance of this high-quality RNA extraction solution in your laboratory.
Reliable and High-Yield RNA Extraction for Bacterial Research
For researchers conducting bacterial RNA studies, having a high-performance RNA extraction kit is essential for obtaining high-quality RNA for transcriptomics, gene expression analysis, and RNA sequencing. The rBAC Mini Total RNA Extraction Kit provides an optimized workflow, ensuring maximum RNA yield and purity in just 30 minutes.
Designed for superior bacterial cell lysis, efficient RNA binding, and contamination-free purification, this extraction kit delivers consistent, high-quality results in microbiology, biotechnology, and molecular biology applications.
Upgrade your bacterial RNA extraction workflow with the rBAC Mini Total RNA Extraction Kit – ensuring purity, speed, and efficiency in every experiment!
- Specifications
- Protocol
- Replacement Columns and Collection Tubes
rBac Specifications:
| Specifications | rBAC Mini Total RNA Kit |
| Format: | Spin Column |
| Binding Capacity: | 50 µg |
| Sample Type: | Gram (+) positive and Gram (-) negative bacterial cells |
| Enzyme: | Lysozyme |
| Typical Yield: | 40-50 µg from 1 x 109Bacterial cells |
| Elution Volume: | 50 µl |
| Operation Time: | <20 mins. |
Protocol Link:
Replacement Columns Link:
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