TAE (Tris / Acetate / EDTA) buffer is commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or current electrophoresis runs making buffer recirculation necessary in such runs. Potential advantages of TAE over TBE buffer include superior resolution of supercoiled DNA and slightly faster migration of double stranded DNA fragments.
- Item Description
- Physical Specifications
- SDS
- Technical Information
Additional Item Description:
The 25 X TAE Buffer Pouch can be mixed with 1 L of ddi water to make 1 L of 25 X TAE Buffer Solution. The pH level does not need to be adjusted.
Key Details:
Specifications:
CAS#:N/A
pH (1 X Solution @ 25ºC):8.2-8.4
Refractive Index (20X Solution):1.330-1.350
SDS Link:
Recommended Use:
TAE buffer systems are commonly used in low voltage DNA electrophoresis applications. It’s relatively low buffering capacity will become exhausted in extended or high current electrophoresis, making buffer recirculation necessary in such runs. Potential advantages of TAE over TBE buffers include superior resolution of supercoiled DNA, and slightly faster migration of ds linear DNA fragments.