gPURE DNA Isolation Kit
Model Number IB47430
- Item Description
- Specifications
- Protocol
- Technical Information
- Sample Size: scalable for cultured cells
- Expectant Yield: high quality (A260/A280=1.8-2.0)
The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Sample:Adherent Cultured Animal cells (trypsinize cells prior to harvesting)
Suspension Cultured Animal cells
Format:Scalable DNA Precipitation Method
Yield:5 – 15 µg
| Cell Number | 0.5-1 x 106 | 3-5 x 106 | 3-5 x 107 |
| Tube Size | 1.5 ml | 15 ml | 15 ml |
| Cell Lysis Buffer | 150 µl | 600 µl | 6 ml |
| RNAse A 10 mg/ml | 1 µl | 3 µl | 30 µl |
| Protein Removal Buffer | 50 µl | 200 µl | 2 ml |
| Isopropanol | 150 µl | 600 µl | 6 ml |
| 70% Ethanol | 150 µl | 600 µl | 6 ml |
| DNA Hydration Buffer | 50 µl | 100 µl | 200 µl |
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