100bp DNA Ladder

100 bp DNA Ladder – Molecular Weight Standard for Electrophoresis

The IBI 100 bp DNA Ladder is a high-quality molecular weight standard designed for sizing double-stranded DNA fragments ranging from 100 base pairs (bp) to 3,000 bp. With 12 linear, double-stranded DNA fragments of defined sizes, this ladder ensures precise and reliable results in agarose gel electrophoresis. Whether you’re conducting routine molecular biology experiments or publishing high-resolution electrophoresis data, the 100 bp DNA Ladder provides unmatched accuracy and convenience.

Key Features of the 100 bp DNA Ladder

- Wide Sizing Range The ladder contains 12 distinct DNA fragments, providing accurate sizing from 100 bp to 3,000 bp. This comprehensive range makes it suitable for diverse molecular biology applications.

- Enhanced 500 bp Fragment The 500 bp band is intensified for quick identification, enabling researchers to easily assess electrophoresis results at a glance.

- Ready-to-Use Format This product comes pre-mixed with 6X loading dye, eliminating the need for dilution. Simply load the sample directly onto your gel and run the electrophoresis.

- Versatile Storage Options The 100 bp DNA Ladder offers versatile storage options, maintaining stability under various conditions. It remains stable at room temperature for up to 6 months, can be stored in refrigeration at 4°C for up to 1 year, and retains its stability when frozen at -20°C for up to 2 years.

- High-Quality Composition The ladder is a unique combination of proprietary plasmids digested with restriction enzymes and PCR products. This ensures consistent performance and accurate molecular weight determination.

Applications of the 100 bp DNA Ladder

- Agarose Gel Electrophoresis Ideal for sizing and quantifying DNA fragments in agarose gel electrophoresis, the 100 bp DNA Ladder provides sharp, clearly defined bands for precise molecular weight estimation.

- PCR Product Analysis The ladder can be used to verify the size of PCR products, ensuring accuracy in applications such as cloning, sequencing, and gene expression studies.

- Quality Control Suitable for assessing the integrity and quality of DNA samples, including plasmids and genomic DNA, during molecular biology workflows.

Product Specifications

- Fragment Sizes: 100 bp, 200 bp, 300 bp, 400 bp, 500 bp (enhanced intensity), 600 bp, 800 bp, 1,000 bp, 1,200 bp, 1,500 bp, 2,000 bp, and 3,000 bp.

- Concentration: 2.5 μg in 25 μL.

- Recommended Loading Volume: 5 μL per lane (0.5 μg DNA).

- Formats Available:

- With Loading Dye: Pre-mixed for convenience (Catalog #: IB01301).

- Without Loading Dye: Allows flexibility in sample preparation (Catalog #: IB01302).

Preparation and Usage Instructions

- Loading: Load 5 μL of the ready-to-use DNA ladder directly onto the agarose gel. No dilution is necessary.

- Electrophoresis: Run the gel under standard electrophoresis conditions appropriate for your gel concentration and buffer system.

- Visualization: Use a UV transilluminator or gel imaging system to visualize the DNA fragments after staining with an appropriate nucleic acid dye (e.g., ethidium bromide or SYBR Safe).

Storage and Stability

To maintain the integrity and performance of the 100 bp DNA Ladder, follow these storage guidelines:

- Store at room temperature (up to 6 months) for short-term use.

- For extended shelf life, store at 4°C (up to 1 year) or -20°C (up to 2 years).

- Avoid repeated freeze-thaw cycles, as they may impact product performance.

Safety Information

The IBI 100 bp DNA Ladder is safe for laboratory use. As with all reagents, handle with care and follow standard laboratory safety practices:

- Wear appropriate personal protective equipment (PPE), including gloves and safety goggles.

- Dispose of any used product according to your institution’s waste disposal guidelines.

- Refer to the Safety Data Sheet (SDS) for additional safety information.

Why Choose IBI’s 100 bp DNA Ladder?

IBI Scientific is a trusted provider of high-quality molecular biology reagents, offering reliable solutions for researchers worldwide. With our 100 bp DNA Ladder, you benefit from:

- Convenience: Pre-mixed with loading dye for immediate use, saving you time and effort.

- Precision: Enhanced intensity at 500 bp ensures quick and accurate size determination.

- Versatility: Suitable for a wide range of applications, from routine gel electrophoresis to advanced molecular biology workflows.

- Trusted Quality: Manufactured with stringent quality control to deliver consistent and reproducible results.

Frequently Asked Questions (FAQs)

1. Can I use this DNA ladder with other gel types, such as polyacrylamide gels? The 100 bp DNA Ladder is optimized for use with agarose gels. While it may work with polyacrylamide gels, performance and band resolution may vary.

2. Is the loading dye compatible with all gel systems? Yes, the included loading dye is compatible with standard agarose gel electrophoresis systems and nucleic acid stains.

3. How should I store the ladder after thawing? After thawing, store the ladder at 4°C for short-term use or return it to -20°C for long-term storage. Avoid repeated freeze-thaw cycles.

4. What’s the benefit of the enhanced 500 bp band? The intensified 500 bp band serves as a reference point, making it easier to quickly interpret electrophoresis results and verify fragment sizes.

5. How many lanes can I run with this sample kit? The sample kit contains enough DNA ladder for 5 lanes (5 μL per lane).

By choosing the IBI 100 bp DNA Ladder, you’re investing in a reliable and accurate molecular weight standard that meets the highest demands of molecular biology research. Order now to simplify your DNA electrophoresis workflows and ensure precise results!