100bp DNA Ladder

100 bp DNA Ladder – Reliable Molecular Weight Standard for Electrophoresis

The IBI 100 bp DNA Ladder is an essential tool for researchers conducting molecular biology experiments that require precise DNA fragment sizing. Designed to serve as a molecular weight standard, this ladder provides accurate and reliable sizing of double-stranded DNA fragments ranging from 100 base pairs (bp) to 3,000 bp. With its 12 distinct fragments and enhanced intensity at the 500 bp marker, the 100 bp DNA Ladder ensures high resolution and ease of use in agarose gel electrophoresis.

Exceptional Accuracy and Performance in DNA Analysis

The IBI 100 bp DNA Ladder offers a broad size range, covering DNA fragments from 100 bp to 3,000 bp. This makes it highly versatile for various molecular biology applications, including PCR product verification, plasmid analysis, and genome mapping. The enhanced 500 bp fragment provides a strong reference point, allowing for quick and easy identification of DNA fragment sizes.

This DNA ladder is manufactured through a precise combination of proprietary plasmids digested with restriction enzymes and PCR-generated fragments. The result is a set of well-defined DNA fragments that migrate consistently during electrophoresis, ensuring accuracy in molecular weight determination.

Pre-Mixed for Convenience and Versatile Storage Options

IBI’s 100 bp DNA Ladder is supplied in a ready-to-use format, pre-mixed with 6X loading dye to streamline laboratory workflows. This eliminates the need for additional preparation steps, allowing researchers to load their samples directly onto the gel. The ladder remains stable under various storage conditions, with the ability to be stored at room temperature for up to six months, refrigerated at 4°C for up to a year, and frozen at -20°C for up to two years. To preserve its performance, repeated freeze-thaw cycles should be minimized.

Ideal for Agarose Gel Electrophoresis and Molecular Biology Applications

The IBI 100 bp DNA Ladder is optimized for use in agarose gel electrophoresis, making it a critical tool for molecular biology research. It enables researchers to accurately determine the size of unknown DNA fragments in PCR analysis, restriction digest mapping, and quality control testing of DNA samples. By providing sharp and clearly defined bands, this DNA ladder enhances resolution and ensures high reproducibility in gel electrophoresis experiments.

This ladder is also widely used in quality control applications, allowing scientists to assess the integrity and purity of their DNA samples. It serves as a reliable benchmark in DNA cloning, sequencing preparation, and various diagnostic applications requiring molecular weight determination. The high-quality composition and strict manufacturing standards of this product guarantee dependable results in all experimental workflows.

How to Use the 100 bp DNA Ladder for Optimal Results

Using the IBI 100 bp DNA Ladder is simple and efficient. Researchers can load 5 μL of the ready-to-use ladder directly onto the agarose gel without requiring dilution. The electrophoresis conditions should be optimized based on the gel concentration and buffer system used. After running the gel, the DNA fragments can be visualized using a UV transilluminator or gel imaging system with an appropriate nucleic acid stain such as ethidium bromide or SYBR Safe. Proper handling and storage ensure the ladder’s continued accuracy and reliability.

Stable and Long-Lasting Performance with Proper Storage

To maintain the integrity of the 100 bp DNA Ladder, it should be stored properly based on the expected duration of use. Short-term storage at room temperature is sufficient for up to six months. For extended stability, refrigeration at 4°C is recommended for up to one year, while long-term storage at -20°C preserves the ladder for up to two years. Minimizing freeze-thaw cycles will help maintain product quality, ensuring consistent and reproducible performance over time.

Safety and Handling Guidelines for Laboratory Use

The IBI 100 bp DNA Ladder is safe for routine laboratory use and does not pose significant health hazards when handled correctly. Standard laboratory safety protocols should be followed, including the use of personal protective equipment such as gloves and safety goggles. Researchers should also adhere to proper waste disposal guidelines when disposing of used gel materials containing nucleic acids.

What Makes IBI’s 100 bp DNA Ladder a Superior Choice?

IBI Scientific is a trusted provider of molecular biology reagents, known for delivering high-quality products that support reliable scientific research. The 100 bp DNA Ladder is manufactured under stringent quality control measures, ensuring batch-to-batch consistency and dependable results in electrophoresis applications. This product provides unmatched convenience with its pre-mixed format, eliminating the need for additional preparation steps and allowing seamless integration into molecular biology workflows.

The enhanced 500 bp fragment is a distinguishing feature of the IBI 100 bp DNA Ladder, providing an intensified band that acts as a reference marker for quick size determination. This feature improves ease of interpretation and accuracy in gel electrophoresis results, making it a valuable tool for researchers analyzing DNA samples of varying fragment sizes.

Frequently Asked Questions About the 100 bp DNA Ladder

Many researchers ask whether this DNA ladder can be used with polyacrylamide gels. While it is primarily optimized for agarose gel electrophoresis, it may be compatible with polyacrylamide gels under specific conditions. Another common question is about the compatibility of the included loading dye with different staining methods. The loading dye is formulated for standard agarose gel electrophoresis systems and works effectively with most nucleic acid stains.

For best storage practices, researchers should store the ladder at 4°C if it is used frequently, or return it to -20°C for long-term preservation. Avoiding repeated freeze-thaw cycles will help maintain the ladder’s integrity and performance. The enhanced 500 bp band is particularly beneficial as a reference marker, allowing quick assessment of electrophoresis results and ensuring precise fragment identification.

The IBI 100 bp DNA Ladder offers researchers an accurate and convenient molecular weight standard, designed to meet the highest standards of molecular biology research. Its precision, ready-to-use formulation, and stable storage options make it the ideal choice for laboratories conducting routine DNA analysis. By using the IBI 100 bp DNA Ladder, researchers can ensure high-resolution electrophoresis results and streamlined workflows, ultimately improving the accuracy and efficiency of their molecular biology experiments.