LB Agar Lennox

Preparation:

To prepare, mix 35 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Other Details:

Physical Specifications:

CAS#: N/A
pH (@ 25°C): 7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications:

Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

LB Agar Lennox Specification Sheet

Certificate of Analysis:

LB Agar Lennox CofA- Lot#19B3014

LB Agar Lennox CofA- Lot#19H5014

LB Agar Lennox CofA -_Lot#20H4025

LB Agar Lennox CofA -Lot#22B3013

LB Agar Lennox CofA -Lot#22H5012

LB Agar Lennox CofA - Lot #23E2004

LB Agar Lennox CofA - Lot#24E2003

SDS Link:

LB Agar Lennox - SDS

Formula per Liter:

● Casein Digest Peptone 10.0 gm
● Sodium Chloride 5.0 gm
● Yeast Extract 5.0 gm
● Agar 15.0 gm

Preparation:

● Mix 35 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use:

IBI’s LB Agar Lennox is used in molecular genetic studies. It is a nutritionally rich media for the growth and maintenance of pure cultures of recombinant strains of E.coli. The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride also provides essential electrolytes. Agar is used as a solidification agent.

Storage:

Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning:

Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.