Maxi Fast-Ion Plasmid Kit
Model Number IB47120
Efficient Maxi Kits for High-Quality Plasmid Purification
The Fast-Ion Maxi Kits are designed for the efficient purification of plasmid or cosmid DNA from bacterial cultures, utilizing pre-packed ion-exchange resin columns for reliable results. This kit employs a modified alkaline lysis method combined with RNase treatment to produce a cleared lysate with minimal genomic DNA and RNA contamination. Through a straightforward gravity-flow procedure, plasmid DNA binds to the column while contaminants are washed away with a specially formulated buffer. The purified DNA is then eluted using a high-salt buffer and precipitated with isopropanol, ensuring a clean, high-yield product in under two hours.
High-Performance Solutions for Plasmid Applications
Purified plasmid DNA from the Fast-Ion Maxi Kits is ideal for applications such as transfection, PCR, sequencing, and in-vitro transcription. Each lot undergoes rigorous quality testing by isolating plasmid DNA from 100 ml of E. coli cultures, with yields exceeding 400 µg per preparation. This ensures consistent, high-quality DNA with excellent performance in downstream applications. The kit supports both high-copy plasmids (100 ml cultures) and low-copy plasmids (250 ml cultures), delivering yields of 500 µg to 1 mg of DNA per prep. With its user-friendly operation and dependable results, the Maxi Kit is an essential tool for plasmid purification.
- Specifications
- Protocol
- Technical Information
- Replacement Columns and Buffers
- Other Plasmid Kits
Specifications Chart:
| Specifications | Maxi Fast-Ion Plasmid Kit |
| Format: | Anion-Exchange |
| Operation: | Gravity Flow |
| Binding Capacity: | 1.5 mg |
| Sample Concentration: | 200-1600 ml |
| Sample Type: | cultured bacterial cells (high-copy = 200-800 ml, low-copy = 500-1600 ml) |
| Fragment Size: | 1-20 kb |
| Typical Yield: | 1-1.5 mg |
| Elution Volume: | 500 µl- 2 ml |
| Operation Time: | <180 minutes |
Protocol Link:
What competitor’s kit is comparable?
QIAGEN Plasmid Maxi Kit QIAGEN QIAfilter Plasmid Maxi Kit
Does this kit come with a plunger?
Our kit does not use a plunger. The Maxi Plasmid uses gravity flow to ensure no plugging will occur and preventing the loss of the sample.
How can I improve the yield from the plasmid kits, when I have low copied DNA?
To improve the DNA yield, increase the volume of bacterial culture to 5 ml, this should work.
What are the new starting volume & yields?
The protocol has been revised as such; the maxi plasmid kits yield up to 1.5 mg of transfection grade plasmid DNA from 400 ml of cultured bacterial cells.
Replacement Column Link:
Check out other Plasmid Kits by IBI:
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.
3. I-Blue Midi Plasmid Kit (Endotoxin-Free)
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
4. Maxi Fast Ion Plasmid Kit (Endotoxin-Free)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
6. I-Blue Mini Plasmid Kit (Endotoxin-Free)
The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns
The High Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures.
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well.
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