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Maxi Fast-Ion Plasmid Kit (Endotoxin-Free) 2 Preps

Maxi Fast-Ion Plasmid Kit (Endotoxin-Free)

Model Number IB47123

$86.65

 

High-Quality Maxi Kits for Efficient DNA Purification

The Maxi Plasmid Kits are designed for efficient and reliable purification of plasmid or cosmid DNA from bacterial cultures. Leveraging pre-packed ion-exchange resin columns, these kits utilize a modified alkaline lysis method paired with RNase treatment to produce a cleared lysate with minimal genomic DNA and RNA contamination. The gravity-flow procedure ensures that plasmid DNA binds effectively to the column, while contaminants are easily washed away using a specialized wash buffer. Purified DNA is eluted using a high-salt buffer and subsequently precipitated with isopropanol for desalting, completing the entire process in under two hours. The resulting DNA is of high quality, making it ideal for applications such as transfection, sequencing reactions, PCR, and in-vitro transcription.

Fast-Ion Maxi Kits: Unmatched Performance and Yield

Every batch of the Fast-Ion Maxi Kits undergoes rigorous lot-to-lot testing to ensure consistent performance. For quality assurance, plasmid DNA is isolated from a 100 ml overnight E. coli (DH5α) culture containing pBluescript, with yields exceeding 400 µg quantified via spectrophotometry. The purified plasmid (1 µg) is tested for integrity using EcoRI digestion and electrophoresis. Designed for convenience and high throughput, these kits handle sample sizes of 100 ml for high-copy plasmids and 250 ml for low-copy plasmids. With an expectant yield of 500 µg to 1 mg of plasmid DNA in just 120 minutes or less, these Maxi Kits deliver reliable and reproducible results for a wide range of molecular biology applications.

  • Specifications
  • Protocol
  • Technical Information
  • Replacement Columns and Buffers
  • Other Plasmid Kits

 Specifications Chart:

Specifications Maxi Fast-Ion Plasmid Kit (Endotoxin-Free)
Format: Anion-Exchange
Operation: Gravity Flow
Binding Capacity: 1.5 mg
Endotoxin Level: <0.10 EU/µg
Sample Concentration: 200-1600 ml
Sample Type: cultured bacterial cells (high-copy = 200-800 ml, low-copy = 500-1600 ml)
Fragment Size: 1-20 kb
Typical Yield: 1-1.5 mg
Elution Volume: 500 µl- 2 ml
Operation Time: <180 minutes

Protocol Link:

Maxi Fast-Ion Endotoxin-Free Plasmid Kit - Protocol

What competitor’s kit is comparable?

QUIGAN EndoFree Plasmid Maxi Kit


What are the new starting volumes and yields?

The protocol has been revised as such: the maxi plasmid kits yield up to 1.5 mg of transfection grade plasmid DNA from 400 ml of cultured bacterial cells.


What is the endotoxin level of eluted DNA in the Maxi Fast-Ion Plasmid Kit?

The endotoxin level is <0.1 EU/µg DNA. This level was determined by LAL testing.

Replacement Column Link:

Replacement Columns and Buffers

Check out other Plasmid Kits by IBI:

      1. Midi Fast Ion Plasmid Kit

The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.

      2. I-Blue Mini Plasmid Kit

The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.

      3. I-Blue Midi Plasmid Kit (Endotoxin-Free)

The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.

      4. High Speed Plasmid Kits

The High Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures. 

      5. I-Blue Midi Plasmid Kit

The I-Blue Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.

      6. I-Blue Mini Plasmid Kit (Endotoxin-Free)

The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns

      7. Maxi Fast Ion Plasmid Kit

The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.

      8. 96-Well Plasmid Kit

The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well.

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