Midi Fast Ion Plasmid Kit
Model Number IB47111
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants.
- Item Description
- Specifications
- Protocol
- Technical Information
- Replacement Columns and Buffers
- Other Plasmid Kits
Additional Item Description:
The Midi Fast Ion Plasmid Kit uses pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.
The quality of the Midi Fast Ion Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing pBluescript (A600>2U/ ml). More than 120 µg of plasmid DNA is quantified with a spectrophotometer. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.
Additional Details:
Specifications Chart:
| Specifications | Midi Fast Ion Plasmid Kit |
| Format: | Anion-Exchange |
| Operation: | Gravity Flow |
| Binding Capacity: | 800 µg |
| Sample Concentration: | 50-300 ml |
| Fragment Size: | 1-20 kb |
| Typical Yield: | 200-800 µg |
| Elution Volume: | 500 µl- 2 ml |
| Operation Time: | <120 minutes |
Protocol Link:
FAQs:
What competitor’s kit is comparable?
QIAGEN Plasmid Maxi Kit or QIAGEN QIAfilter Plasmid Maxi Kit
What is the formulation for the PW buffer in the Midi plasmid kit?
This is not exact, but it is QIAGAN’s formulation for a similar kit. The formulation will work fine. 1.0 M NaCl, 50 mM MOPS, pH 7.0 15% Isopropanol.
Replacement Column Link:
Check out other Plasmid Kits by IBI:
1. High-Speed Mini Plasmid Kit
The High Speed Mini Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-5ml of bacterial cultures.
The I-Blue Mini Plasmid Kit represents a cutting-edge solution tailored for the swift and efficient isolation of plasmid DNA from 1-7 ml of cultured bacterial cells.
3. I-Blue Midi Plasmid Kit (Endotoxin-Free)
The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
4. Maxi Fast Ion Plasmid Kit (Endotoxin-Free)
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The I-Blue Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells.
6. I-Blue Mini Plasmid Kit (Endotoxin-Free)
The I-Blue Mini Plasmid Endo-Free Kit was designed for transfection grade plasmid DNA purification from 1-5ml of cultured E.coli cells using silica-membrane spin columns
The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures.
The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well.
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