Proteinase K Solution

Additional Item Description:

IBI Scientific recombinant Proteinase K Powder is highly pure, determined by SDS-PAGE to be 95%.

High purity, sterility, no bio-burden and no presence of DNase, RNase, DNA, and RNA contaminants.

By using absorbance A275 as the vertical axis and different concentrations of a tyrosine as the horizontal axis the enzymatic activity was calculated to >30U/mg.

IBI Scientific recombinant Proteinase K Powder is expressed in pichia pastoris, purified and lyophilized. 

Physical Specifications:

Molecular Weight = 28.9 kda
Enzyme Activity = >30U/mg
DNase Assay = None Detected
RNase Assay = None Detected
Size: 5 ml
Concentration: 20 mg/ml

Storage:

• -20C for up to 2 years
• 4C for up to 18 months
• Room temperature for up to 12 months
• Is best to make aliquots for multiple uses
• Avoid repeated freeze-thaw cycles

IB05406 - Specification Sheet

Certificate of Analysis:

CofA_IB05406_-_Lot#19DC1217

CofA_IB05406_-_Lot#19DC1022

 CofA IB05406 - Lot#21DC1012C

SDS Link:

How This Solution Was Manufactured:

• 50mM Tris
• 3mM CaCl2
• 50% Glycerol
• Adjust pH to 8.0 with either HCl or NaOH

1. Dissolve solids in 50% volume of Certified Nuclease Free Water (IB42100)
2. QS to 95% with glycerol. pH to 8.0 using HCL or NaOH.
3. QS to 100% volume with glycerol.
4. Recheck pH using different meter.
5. Mix well using a sterile stir bar and filter through a 0.2 micron sterile filter.
6. Store buffer at 4C and let chill before preparing Proteinase K solution.

Using cold buffer, remove Proteinase K from freezer and dilute powder to 10mg,mL with buffer. Mix well using a stir bar. Filter solution through a 1.2 micron PES membrane syringe or disc filter and aseptically fill into final container. Aliquot and store at -20C.