Terrific Broth
Model Number IB49140
IBI’s Terrific Broth is used with Glycerol in cultivating recombinant strains of E. coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume.
- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
- Technical Information
- Citations
Additional Item Description:
Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.
Key Details:
Physical Attributes:
CAS#: N/A
pH (@ 25°C): 7.2 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.
Molecular Biology Specifications:
Escherichia coli ATC 23724: Growth
Escherichia coli ATC 33694: Growth
Escherichia coli ATC 33849: Growth
Escherichia coli ATC 39403: Growth
Escherichia coli ATC 47014: Growth
Escherichia coli ATC 53868: Growth
Certificate of Analysis Links:
Terrific Broth CofA- Lot#19B3024Terrific Broth CofA- Lot#19G5021
Terrific Broth CofA -_Lot#20A3015
Terrific Broth CofA -_Lot#20C2003
Terrific Broth CofA_-_Lot#20G4001
Terrific Broth CofA_-_Lot#20J3002
Terrific Broth CofA_-_Lot#21C2012
Terrific Broth CofA - Lot#21E2005
Terrific Broth CofA - Lot#21H1003
Terrific Broth CofA - Lot#21H4011
Terrific Broth CofA - Lot#21H4001
Terrific Broth CofA - Lot#22B1002
Terrific Broth CofA - Lot#22C1012
Terrific Broth CofA - Lot#22C1002
Terrific Broth CofA - Lot#21L5041
Terrific Broth CofA - Lot#22F3002
Terrific Broth CofA - Lot#22L1012
Terrific Broth CofA - Lot#23C1003
Terrific Broth CofA - Lot#23D1014
Terrific Broth CofA - Lot#23D3005
Terrific Broth CofA - Lot#23H3013
Terrific Broth CofA - Lot#24C5004
SDS Links:
Formula per Liter:
● Casein Digest Peptone 12.0 gm
● Yeast Extract 24.0 gm
● Potassium Phosphate, Monobasic 2.3 gm
● Potassium Phosphate, Dibasic 12.5 gm
Preparation:
● Mix 50.8 gm of the medium in 1 L of purified water until evenly dispersed.
● Add 4 ml of Glycerol to the mixture.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.
Recommended Use:
IBI’s Terrific Broth is used with Glycerol in cultivating recombinant strains of E.coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E.coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.
Storage:
Store at room temperature. Keep tightly sealed. Protect from moisture and light.
Warning:
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.
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Article Link: Read Article
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