Blood Base Agar #2

• Used for cultivation of a wide variety of microorganisms

IBI’s Blood Agar Base #2 is recommended for the cultivation of a wide variety of microorganisms. When prepared with 5 – 10% blood, the media can be used to cultivate fastidious microorganisms and study hemolytic reactions.

Store at room temperature (less than 30⁰C), tightly sealed, and protect from moisture and light.

Cas#: N/A
pH @ 25°C: 7.4 ±0.2
Note:Grams per/liter may be adjusted to achieve desired performance.

Molecular Biology Specifications
Eschericha coli ATCC 25922: Growth Straphylococcus ATCC 25923: Growth Streptococcus pneumonia ATCC 6305: Growth Streptococcus pyogenes ATCC 19615: Growth

Blood Base Agar #2 Specification Sheet

Blood Base Agar #2 CofA - Lot#17K5040

Blood Base Agar #2 CofA - Lot# K15-01

Blood Base Agar #2 CofA - Lot# J11-04

Blood Base Agar #2 CofA - Lot# C11-64

Blood Base Agar #2 CofA - Lot# 18F2023

Blood Base Agar #2 CofA - Lot# 18G4035

Blood Agar Base #2 - SDS

Formula per Liter
● Casein Digest Peptone 7.5 gm
● Peptic Digest of Animal Tissue 7.5 gm
● Sodium Chloride 5.0 gm
● Liver Digest 5.0 gm
● Yeast Extract 5.0 gm
● Agar 12.0 gm

Preparation
Mix 39.5 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and boil
for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Prepare 5 to 10% blood
agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C.
Dispense into Petri dishes or tubes.

Recommended Use
Blood Agar Base #2 is recommended for the cultivation of a wide variety of microorganisms. When prepared with 5
to 10% blood, the media can be used to cultivate fastidious microorganisms and study hemolytic reactions.

Storage
Store at room temperature (less than 30°C). Keep tightly sealed. Protect from moisture and light.

Warning
Avoid contact to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face
protection. See Material Safety Data Sheet for additional information.