Millers LB Agar

• Contains casein digest peptone, sodium chloride, yeast extract, and agar
• For maintaining and propagating E. coli culture

IBI’s Miller LB Agar is used in molecular genetic studies, as well as maintaining and propagating E. coli in molecular and microbiology procedures. The inclusion of casein peptone and yeast extract provides essential growth factors for the bacteria; such as nitrogen, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes. Agar is used as a solidification agent.

To prepare, mix 40 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Physical Specifications
CAS#:N/A
pH (@ 25°C):7.0 ± 0.2
NOTE:Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications
Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

Millers LB Agar Specification Sheet

Millers LB Agar CofA - Lot# 18C1004

Millers LB Agar CofA - Lot# 16K4010

Millers LB Agar CofA - Lot# 16G4006

Millers LB Agar CofA - Lot# 160211012002

Millers LB Agar CofA - Lot# H15-04

Millers LB Agar CofA - Lot#E15-42

Millers LB Agar CofA - Lot# C14-41

Millers LB Agar CofA - Lot# L13-05

Millers LB Agar CofA - Lot# 18F5005

Millers LB Agar CofA- Lot#19B3004

Millers LB Agar CofA - Lot#19E2005

Millers LB Agar CofA_-_Lot#20H1013

Millers LB Agar CofA - Lot#21K5021
Millers LB Agar CofA - Lot #22L4011

Millers LB Agar CofA - Lot#22A1004

Millers LB Agar CofA - Lot#23F3001

Millers LB Agar CofA - Lot#23M3002

Millers LB Agar CofA - Lot#24H5024

Millers LB Agar - SDS

Formula per Liter
● Casein Digest Peptone 10.0 gm
● Sodium Chloride 10.0 gm
● Yeast Extract 5.0 gm
● Agar 15.0 gm

Preparation
● Mix 40 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use
IBI’s Luria Agar is used in molecular genetic studies, as well as maintaining and propagating E.coli in molecular and microbiology procedures. The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes. Agar is used as a solidification agent.

Storage
Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning
Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.