10X TBE Buffer

TBE Buffer: Superior Buffering Capacity for Electrophoresis Applications

TBE (Tris / Boric Acid / EDTA) buffer is a high-performance solution designed to offer superior buffering capacity compared to TAE. With its enhanced ability to maintain pH stability over extended electrophoresis runs, TBE is the preferred choice for DNA sequencing, gel electrophoresis, and other applications requiring high-resolution separation. This buffer eliminates the need for recirculation in most electrophoresis protocols, making it more efficient and reliable for laboratories conducting extended gel runs.

Exceptional Buffering Power

TBE buffer provides a significantly greater buffering capacity than TAE, ensuring that nucleic acids remain stable and well-separated throughout electrophoresis. The inclusion of Tris, Boric Acid, and EDTA enables TBE to maintain a stable pH between 8.1 and 8.5, which is critical for consistent results. This pH stability reduces band distortion and enhances resolution, making it ideal for applications that require precision, such as DNA sequencing and Northern/Southern blotting.

Key Advantages of TBE Buffer

TBE buffer maintains a stable pH longer than TAE, preventing shifts that can affect DNA migration. Due to its strong buffering ability, it eliminates the need for recirculating buffer, except in extremely long runs. It delivers sharp, clear bands, improving the accuracy of nucleic acid analysis. Ideal for applications requiring long electrophoresis times, TBE ensures consistent results over prolonged periods. The presence of EDTA chelates divalent cations, reducing the risk of nucleic acid degradation by nucleases.

Product Specifications

This buffer consists of Tris, Boric Acid, and EDTA at a concentration of 10X. The pH range is between 8.1 and 8.5 and can be adjusted with ddi water as needed. It comes in a powdered form in a ready pouch, with each pouch making one liter of 10X TBE buffer solution.

Simple Preparation and Usage

Preparing TBE buffer is quick and straightforward. The 10X TBE Buffer Ready Pouch allows users to conveniently create a high-quality electrophoresis buffer with minimal effort. To prepare the solution, dissolve the contents of one 10X TBE Buffer Ready Pouch in ddi water, adjust the final volume to one liter, ensure the pH is between 8.1 and 8.5 for optimal electrophoresis performance, and use it at the required dilution (e.g., 0.5X or 1X) based on experimental needs.

Applications of TBE Buffer

TBE buffer is widely used in molecular biology and biotechnology research, particularly in applications requiring high-resolution separation of nucleic acids. It is commonly used in agarose gel electrophoresis for separating DNA and RNA fragments with superior clarity. It is also ideal for polyacrylamide gel electrophoresis (PAGE), which provides high-resolution separation of smaller nucleic acid fragments. Additionally, it delivers consistent and precise results for DNA and RNA sequencing. In Northern and Southern blotting, it ensures high-quality transfer of nucleic acids during hybridization procedures. Furthermore, it is useful for checking PCR amplification products before downstream applications.

Why Choose TBE Buffer Over TAE?

While both TBE and TAE are commonly used in electrophoresis, TBE offers distinct advantages that make it superior for specific applications. TBE maintains its pH longer than TAE, making it the preferred choice for extended electrophoresis runs. The high ionic strength of TBE allows for sharper, more distinct DNA bands, improving resolution. With minimal buffer depletion, TBE supports longer gel run times without requiring frequent buffer changes. Additionally, the EDTA in TBE inhibits enzymes that degrade DNA, preserving sample integrity.

Storage and Stability

TBE buffer is highly stable when stored properly. Keep the buffer in a cool, dry place, away from direct light and moisture. Once prepared, store the solution at room temperature or in the refrigerator to maintain its effectiveness for extended periods.

Optimize Your Electrophoresis Performance with TBE Buffer

For researchers and laboratories looking for a reliable, high-performance electrophoresis buffer, TBE is the ideal solution. With its superior buffering capacity, long-lasting pH stability, and ease of use, TBE buffer ensures accurate and reproducible results for DNA and RNA electrophoresis applications.

Order Your 10X TBE Buffer Ready Pouch Today!

Ensure superior performance in your electrophoresis applications by choosing TBE buffer. Contact us for bulk orders, product inquiries, or additional technical support. Experience the benefits of high-quality TBE buffer and take your nucleic acid analysis to the next level.