X-Gal

CAS# 7240-90-6

• Used as a histochemical substrate for β-galactosidase which allows the detection of lac gene expression

X-GAL is a histochemical substrate for β-galactosidase which allows the detection of lac gene expression.

To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage from light and stored at -20⁰C.

CAS# 7240-90-6
Molecular Formula C₁₄H₁₅BrClNO₆
Molecular Weight 408.64
Purity Min. 99%
Solubility (2%, in N,N’ DMF) Clear and colorless
Molecular Biology Specifications
Optical rotation (C=1) - Pass

IB02264 - Specification Sheet

CofA - Lot#17G0156

CofA - Lot#15G1250

CofA - Lot#13L0250

CofA_-_Lot#20L3003

CofA_-_Lot#21A3005

IB02264 - SDS

Recommended Use A histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage by light and should be stored at –20°C. (It is not necessary to sterilize X-GAL solutions by filtration.)

Storage Store desiccated at –20°C. Warm to room temperature before opening. Keep tightly sealed.

What can X-Gal be dissolved in or re-suspended in? Dimethylformamide (DMF) and Dimethyl sulfoxide (DMSO)

How long can this be out of the refrigerator? Not more than 4-6 hours. *If solution turns pink, discard.