
Glycine (CAS# 56-40-6) for Polyacrylamide Buffer Systems
Glycine is a neutral amino acid widely used in electrophoresis and biochemical research. As a key component in polyacrylamide buffer systems, glycine plays a critical role in protein and nucleic acid separation techniques, including SDS-PAGE and native PAGE. During electrophoresis, glycine migrates to form an electrical front, ensuring efficient macromolecule separation by facilitating proper protein stacking and migration.
Buffer Preparation and Biochemical Applications
IBI's Glycine is commonly used to prepare concentrated stock solutions for laboratory applications. It functions as a buffering agent in biochemical and cell culture environments, helping to maintain stable pH conditions. Its zwitterionic properties make it effective in regulating pH in techniques such as Western blotting and protein purification.
This high-purity glycine is ideal for research and laboratory use, supporting reliable results in protein and nucleic acid analysis. It is an essential reagent in molecular biology and biochemistry, ensuring accuracy in electrophoresis and buffer preparation.
- Physical Specifications
- Certificate of Analysis
- SDS
Physical Specifications:
CAS#:56-40-6
Formula Weight:75.07
Molecular Formula:C2H5NO2
Purity:>99.5%
A280 (1 M, water):<0.10
Heavy Metals (as Pb) (%):<0.002%
Solubility (10% water):Pass
pH (10% water):5.5-6.5
Loss on Drying (%):≤0.2
Melting Point:247±2.5° C
Molecular Biology Specifications:
DNase Assay:None Detected
RNase Assay:None Detected
Protease Assay:None Detected