NU-CLEAN G50 w/STE

• Retention of unincorporated NTPs: up to 99%
• Recovery of DNA (˃72bp): ˃90%
• Operation time: up to 10 minutes
• DNase- and RNase-free

The Nu-Clean D50/Sephadex, G-50 spin columns are intended for use in desalting and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from end-label, fill-in, nick-translation, or random primed DNA labeling reactions. This column should be used to purify DNA greater than 72 base pairs in length or RNA greater than 72 base length; smaller DNAs or RNAs will be retained in the column material. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume. For optimum sample recovery and purification when preparing biotinylated probes, use Nu-Clean spin columns with the “B” designation.

Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile STE buffer. Two autoclavable collection tubes and one non-autoclavable cap are supplied for each column. Product requires a swing bucket centrifuge rotor.

The optimal sample loading volume is 50µl (100µl Maximum) with a maximum of 100µg of nucleic acid per column.

Sample Size:Up to 100ul
Format:Gel filtration in spin column
Carrier Buffer:STE
G50 Sephadex inside spin column containing frit filter. Column capped on both ends.

IB06050-Spin Column Protocol