- Item Description
- Product Video
- Specifications / Certificate of Analysis
- SDS
- Sterile filled in 2ml vials (screw cap)
- Packaged in 20, 50 & 100 packs
- Nuclease FREE water determined by fluorometric analysis
- Endotoxin FREE water determined by kinetic chromogenic LAL method as specified in USP <85> or EP <2.6.14>. Detection to 0.005 EU/ml
IBI Scientific PCR Water is ideal for use in all PCR reactions such qPCR, RT-PCR, and RT-qPCR and conveniently packaged in 2ml vials that fit perfectly into your reaction set up work flow. Small vials also reduce waste in the case of accidental contamination.
IBI Scientific PCR water is developed using the latest in water purification technology including reverse osmosis, EDI, UV Light and ultra-filtration all built into one large system. The EDI module contains special resins to deionize the water and achieve 18 meg-ohm of resistivity. The water then passes through a 0.2 micron filter before being exposed to a specially tuned UV light frequency that will oxidize all organic material. After UV oxidation the water passes through an ultra-filtration membrane which removes all oxidized organics and renders the bacterial endotoxin levels in the water to less than 0.005 EU/ml. Ultrafiltration also produces Nuclease Free Water, meaning No DNase, RNase or Protease.
Water samples from this system are regularly tested and validated by 2 independent laboratories testing for nuclease activity and bacterial endotoxin load. IBI internal quality control methods are tested and validated against the results of the independent laboratories using same samples.
The document below serves as a specification sheet and certificate of analysis for all PCR Grade water products
PCR Grade Water - Analysis Sheet
Specifications For PCR WaterGrade | PCR Grade Water |
Cations & Anions Free: | Yes |
Mycoplasma Free: | Yes |
Sterility Assurance By: | 0.1 µm pore filter |
Resistivity: | 18.2 mOhms |
Endotoxin (LAL): | ≤ 0.1 EU/ml |
Electrophoresis: | Yes |
PCR: | Yes |
RT-PCR: | Yes |
MEA Approved: | N/A |
RNAse: | None Detected |
DNAse: | None Detected |
Nickase: | None Detected |
Protease: | None Detected |
Phosphatase: | None Detected |
Mycoplasma: | None Detected |
Iron (Fe): | Not Tested |
Sterility Assured: | 0.1 µm sterile-filtered and 2 X autoclaved |
Available Bottle Sizes: | 20 X 1.8 ml Vials 50 X 1.8 ml Vials 100 X 1.8 ml Vials |