- Item Description
- Physical Specifications
- Certificate of Analysis
- SDS
- Technical Information
- Ready to use IPTG/X-gal solutionfor selection of cloning construct in LacZ expression in the presence of lacl repressor
- Concentration: 8mg/ml
- Each spray produces approximately 0.20ml
- Proprietary formulation enhances blue color
IPTG/X-GAL Solution is a ready-to-use product packaged in an easy-to-use spray bottle. The solution is used in selection of cloning construct in LacZ expression in the presence of lacl repressor.
The concentration of this solution is 8 mg/ml of IPTG and X-GAL. Each pump of the easy to use spray bottle produces approximately 0.20 ml of solution. This volume of solution per actuation should be used to determine your required volume per plate. Spread solution and pause to ensure absorption by waiting approximately 5 to 10 minutes. IBI IPTG/X-gal solution is the only one on the market that can be kept at room temp for extended periods of time without harming the performance of the solution. Storage at 4 deg C is recommended for long term. Made without DMSO. This proprietary formulation enhances the blue color thus making colony picking much easier! Better contrast and less false positives.
Physical Specifications
CAS#: N/A
Solution Components: Isopropyl -D-thiogalatopyranoside 5’ bromo-4-chloro-3-indolyl-D-galactopyranoside Appearance: Clear, colorless to yellow solution
Molecular Biology Specifications
Does not contain toxic DMF and DMSO.
To obtain CofA for this item, please call 800-253-4942.
Thank you
Recommended Use
IBI’s IPTG / X-GAL Solution is a ready-to-use product packaged in an easy-to-use spray bottle. For use in selection of cloning construct in LacZ expression in the presence of lacl repressor. The concentration of this solution is 8 mg/ml of IPTG and X-GAL. Each pump of the easy to use spray bottle produces approximately 0.20 ml of solution. This volume of solution per/actuation should be used to determine your required volume per plate. Spread solution and pause to ensure absorption, waiting approximately 5 to 10 minutes.
Storage
Store at +4°C. Keep tightly sealed.