LB Miller Broth

Physical Specifications:

CAS#:N/A
pH (@ 25°C):7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications:

Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

LB Miller Broth Specification Sheet

What Does LB Stand for in Broth?

LB stands for Luria-Bertani, named after its creators, Giuseppe Bertani and Salvador Luria. It is a nutritionally rich medium widely used for the growth of bacteria, particularly Escherichia coli (E. coli), in microbiology and molecular biology laboratories.

What is the Purpose of the LB Broth in the Tubes?

The purpose of LB broth in tubes is to provide a nutrient-rich environment for the growth and maintenance of bacterial cultures. The broth contains all the essential nutrients, such as peptides, amino acids, vitamins, and minerals, that bacteria need to grow and thrive. LB broth in tubes is often used for:

• Culturing Bacteria: Growing bacterial cultures for various experiments and analyses.
• Maintaining Stocks: Preserving bacterial strains for future use.
• Plasmid Amplification: Supporting the growth of bacteria carrying plasmids for genetic studies.

Why is LB Broth Good for E. coli?

LB broth is particularly good for E. coli due to its nutrient composition, which supports rapid growth and high cell density. Key reasons include:

• Nutrient-Rich: LB broth provides a balanced mixture of nutrients, including peptone, yeast extract, and sodium chloride, which are essential for the growth and metabolism of E. coli.
• Optimal pH: The pH of LB broth is well-suited for E. coli, ensuring an ideal environment for its proliferation.
• Versatility: LB broth can be used for a variety of applications, from cloning and protein expression to routine culturing.

Is LB Broth the Same as Nutrient Broth?

LB broth and nutrient broth are both used for culturing bacteria, but they have different compositions and applications:

• LB Broth:

  • Composition: Contains tryptone, yeast extract, and sodium chloride.
  • Use: Primarily used for the growth of E. coli and other enteric bacteria in molecular biology.

• Nutrient Broth:

  • Composition: Contains beef extract, peptone, and sometimes sodium chloride.
  • Use: Used for the general cultivation of a wide range of non-fastidious organisms.

While both broths support bacterial growth, LB broth is specifically optimized for molecular biology applications, particularly those involving E. coli.

Benefits of Using LB Broth

1. Rapid Growth: Supports fast and robust growth of E. coli and other enteric bacteria.
2. Versatile Applications: Suitable for a wide range of microbiological and molecular biology experiments.
3. Consistency: Provides a reliable and consistent environment for reproducible results.

Incorporating LB broth into your laboratory protocols ensures optimal growth conditions for your bacterial cultures, making it an essential tool in microbiology and molecular biology research.

Certificate of Analysis Link:

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SDS Link:

LB Miller Broth - SDS

Formula per Liter:

● Casein Digest Peptone 10.0 gm
● Sodium Chloride 10.0 gm
● Yeast Extract 5.0 gm

Preparation:

● Mix 25 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use:

IBI’s Luria Broth (LB Miller) is used in molecular genetic studies, as well as maintaining and propagating E.coli in molecular and microbiology procedures. The inclusion of casein peptone and yeast extract supply essential growth factors; such as nitrogen, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

Storage:

Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning:

Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.