MINI Flex Tube Kit, 25KDa MWCO

IBI MINI FlexTubes for Dialysis and Gel Extraction

The IBI MINI FlexTubes are designed for precise small-volume applications, including dialysis, gel extraction, electro-elution, and de-salting. With a Molecular Weight Cut-Off (MWCO) of 25KDa and a membrane size of 0.4cm x 1.1cm, these tubes efficiently isolate proteins, DNA, RNA, and oligonucleotides (>15nt) in volumes ranging from 10-250µl. The regenerated cellulose membrane ensures excellent sample recovery, while the screw-cap top allows seamless loading and unloading using a standard pipette tip. Multiple FlexTubes can be placed in one buffer tank, enabling high-throughput electro-elution with minimal hands-on time. 

For optimal protein gel extraction, the IBI MINI FlexTubes should be used with Quick Blue Protein Stain, ensuring high recovery yields without protein fixation or the need for hazardous solvents. 

High Recovery Yields and Enhanced Purity

The IBI MINI FlexTubes deliver exceptional recovery yields, with up to 70% for proteins and over 90% for DNA fragments from agarose or polyacrylamide gels. Large DNA fragments (>100Kb) and protein-nucleic acid complexes are easily extracted, while the tube’s compatibility with SDS removal enhances sample purity for downstream applications like MALDI-MS. For mass spectrometry analysis, the use of MS Buffer enables efficient protein precipitation, ensuring greater sensitivity and accurate molecular mass determination. Compared to traditional diffusion methods, the FlexTube simplifies de-salting and Oligo PAGE electro-elution, offering a faster and more efficient solution. Rigorously tested for gel extraction, dialysis, and buffer exchange, the IBI MINI FlexTubes ensure high-quality results for molecular biology workflows.