Proteinase K Powder
Model Number IB05400
$97.60
- Physical Specifications
- Certificate of Analysis
- SDS
- Technical Information
Physical Specifications:
Molecular Weight = 28.9 kda
Enzyme Activity = >30U/mg
DNase Assay = None Detected
RNase Assay = None Detected
Form: Lyophilized Powder
Storage:
- -20C for up to 2 years
- 4C for up to 18 months
- Room temperature for up to 12 months
- Is best to make aliquots for multiple uses
- Avoid repeated freeze-thaw cycles
SDS Link:
To place this product into solution:
- 50mM Tris
- 3mM CaCl2
- 50% Glycerol
- Adjust pH to 8.0 with either HCl or NaOH
- Dissolve solids in 50% volume of Certified Nuclease Free Water (IB42100)
- QS to 95% with glycerol. pH to 8.0 using HCL or NaOH.
- QS to 100% volume with glycerol.
- Recheck pH using different meter.
- Mix well using a sterile stir bar and filter through a 0.2 micron sterile filter.
- Store buffer at 4C and let chill before preparing Proteinase K solution.
Using cold buffer, remove Proteinase K from freezer and dilute powder to 10mg,mL with buffer. Mix well using a stir bar. Filter solution through a 1.2 micron PES membrane syringe or disc filter and aseptically fill into final container. Aliquot and store at -20C.
- Use containers and materials that have low protein binding characteristics.
Note: If you are able to use this product entirely in 15 days or less, IBI Recombinant Proteinase K Powder can be placed directly into Pure water and 50% glycerin at required concentration.
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