Yeast Nitrogen Base (Without Amino Acids)
Model Number IB49220
What is Yeast Nitrogen Base?
Yeast Nitrogen Base (YNB) without amino acids is a widely used broth medium for culturing yeasts, particularly for classification based on amino acid and carbohydrate requirements. This medium includes essential vitamins, inorganic salts, and trace elements necessary for yeast growth, except for histidine, methionine, tryptophan, and a carbohydrate source. Its unique formulation makes it an ideal choice for studying yeast metabolism and genetic manipulation.
IBI Scientific Yeast Nitrogen Base
IBI Scientific Yeast Nitrogen Base w/o amino acids is specifically designed for precise yeast classification and experimentation. Free of amino acids, this nutrient-rich medium supports the growth of wild-type yeast and enables selectable marker utilization in cloning and yeast artificial chromosome manipulation. With its high-quality formulation, IBI Scientific ensures reliable and reproducible results for researchers working in microbiology and molecular biology.
- Product Specifications
- Certificate of Analysis
- SDS
- Technical Information
Product Link:
SDS Link:
Formula g/L
| Ammonium Sulfate | 5.0 g | Calcium Pantothenate | 0.4 mg | |
| Biotin | 2.0 ug | Inositol | 2.0mg | |
| Folic Acid | 2.0 ug | p-Aminobenzoic Acid | 0.2mg | |
| Niacin | 0.4 mg | Riboflavin | 0.2mg | |
| Pyridoxine Hydrochloride | 0.4 mg | Boric Acid | 0.5 mg | |
| Thiamine Hydrochloride | 0.4 mg | Potassium Iodide | 0.1 mg | |
| Copper Sulfate | 40.0 ug | Manganese Sulfate | 0.4 mg | |
| Ferric Chloride | 0.2 mg | Zinc Sulfate | 0.4 mg | |
| Sodium Molybdate | 0.2 mg | Magnesium Sulfate | 0.5 g | |
| Monopotassium Phosphate | 1.0 g | Calcium Chloride | 0.1 g | |
| Sodium Chloride | 0.1 g |
Final pH: 5.4 +/- 0.2 at 25C
Preparation
Prepare a 10X solution by dissolving 6.7 g of the base, 5 g of dextrose or equivalent amount of other carbohydrate and 5 – 10 ml of the desired amino acid in 100 ml of purified water until evenly dispersed. Warm slightly in a 45-50 C water bath with repeated stirring to dissolve.
DO NOT BRING TO A BOIL. DO NOT AUTOCLAVE.
Mix well and filter-sterilize. Store at 2-8 C. Prepare the final medium by aseptically adding 0.5ml of the 10X solution to 4.5 ml of sterile water. Mix the solution by shaking gently prior to inoculation.
Quality Control Specifications
- The powder is homogenous, free flowing and off-white.
- Visually the prepared medium is clear and almost colorless.
- Expected culture response after 3 – 5 days at 30 C.
Organism | Result |
Candida Albicans ATCC 10231 | Good Growth |
Kloeckera apiculata ATCC 9774 | Good Growth |
Saccharomyces cerevisiae ATCC 9080 | Good Growth |
Saccharomyces cerevisiae ATTC 9763 | Good Growth |
Storage
Store the sealed bottled containing the dehydrated medium at 2 – 30 C. Once opened and re-capped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or of the color has changed from the original color.
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