Terrific Broth

Terrific Broth – High-Yield Enriched Medium for E. coli Cultivation

IBI’s Terrific Broth is a nutrient-rich, high-performance medium designed to optimize the growth and plasmid yield of Escherichia coli (E. coli) cultures. Its specialized formulation ensures an extended growth phase, enabling the cultivation of high-density bacterial cultures with significantly improved plasmid production. Whether you’re conducting molecular biology research, protein expression studies, or industrial plasmid production, Terrific Broth is the ideal choice for achieving superior results.

Key Features of Terrific Broth

1. Rich in Essential Nutrients Terrific Broth contains high concentrations of tryptone and yeast extract, two critical components that provide a robust source of amino acids, peptides, vitamins, and other growth factors. These nutrients are essential for supporting robust bacterial growth and optimizing plasmid yields.

2. Glycerol as a Carbon Source Unlike glucose-based media, Terrific Broth utilizes glycerol as its primary carbon source. This minimizes the accumulation of acetic acid, a byproduct that can inhibit bacterial growth and reduce plasmid yields. By maintaining a more favorable metabolic environment, the medium promotes healthy and sustained bacterial proliferation.

3. Optimized Buffering System The addition of potassium phosphates in Terrific Broth helps maintain a stable pH throughout the bacterial growth cycle. This buffering system prevents harmful pH fluctuations that can compromise the efficiency of recombinant E. coli cultivation, ensuring consistent and reliable results.

Applications of Terrific Broth

Terrific Broth’s advanced formulation makes it suitable for a wide range of laboratory and industrial applications:

1. Recombinant E. coli Cultivation Designed specifically for cultivating recombinant strains of E. coli, Terrific Broth supports extended growth phases and higher cell densities, providing a reliable foundation for molecular biology and genetic engineering experiments.

2. High-Yield Plasmid Production Terrific Broth excels in applications requiring high plasmid yields, making it an invaluable tool for DNA extraction, cloning, and other plasmid-based research. Its nutrient-rich profile ensures a high plasmid copy number per volume.

3. Protein Expression Studies For researchers focused on recombinant protein expression, Terrific Broth’s ability to support high-density cultures translates into greater protein yield. This makes it an excellent choice for downstream applications such as protein purification and functional analysis.

4. Industrial and Academic Research Whether you’re working in an academic laboratory or a biotechnology production facility, Terrific Broth provides consistent, reproducible results across a variety of workflows.

Preparation Instructions for Terrific Broth

To prepare 1 liter of Terrific Broth:

1. Dissolve 47.6 grams of Terrific Broth powder in 900 mL of distilled water.

2. Add 4 mL of glycerol to the solution.

3. Adjust the total volume to 1 liter with distilled water.

4. Sterilize the prepared medium by autoclaving at 121°C for 15 minutes.

5. Allow the medium to cool to room temperature before inoculating bacterial cultures.

By following these preparation instructions, you can ensure optimal performance and reproducibility in your experiments.

Storage and Stability

- Storage Conditions: Store the dehydrated Terrific Broth powder in a tightly sealed container at room temperature. Protect the product from moisture and light to maintain its integrity.

- Shelf Life: Proper storage conditions ensure that the product remains stable and effective over an extended period, providing reliable results for all your bacterial culture needs.

Safety Information

Terrific Broth is classified as an irritant and should be handled with care. Follow these safety guidelines to minimize risk:

1. Wear appropriate personal protective equipment (PPE), including gloves, safety goggles, and a lab coat.

2. Avoid inhalation, ingestion, or direct contact with the skin and eyes.

3. In case of accidental contact, rinse the affected area thoroughly with water and seek medical advice if necessary.

4. Consult the Safety Data Sheet (SDS) for comprehensive safety and handling instructions.

Why Choose IBI’s Terrific Broth?

IBI Scientific is committed to providing high-quality laboratory reagents and culture media that meet the rigorous demands of scientific research. Our Terrific Broth is manufactured with precision to ensure consistency, reliability, and optimal performance in every batch. By choosing IBI’s Terrific Broth, you can:

- Achieve higher plasmid yields with recombinant E. coli cultures.

- Support high-density bacterial growth for protein expression and molecular biology studies.

- Rely on a product that meets industry standards for purity and performance.

Frequently Asked Questions (FAQs)

1. What makes Terrific Broth different from other media? Terrific Broth is enriched with higher concentrations of nutrients and uses glycerol instead of glucose, reducing acetic acid buildup. This results in improved plasmid yields and healthier bacterial cultures.

2. Can I use Terrific Broth for non-recombinant E. coli strains? Yes, while it is optimized for recombinant strains, Terrific Broth can support the growth of non-recombinant E. coli and other bacterial species that thrive in nutrient-rich environments.

3. How should I store prepared Terrific Broth? Store sterilized Terrific Broth in sterile containers at 4°C for short-term use. Avoid contamination by using aseptic techniques.

4. Is Terrific Broth suitable for industrial-scale applications? Absolutely! Terrific Broth’s reproducibility and performance make it ideal for both research and large-scale production workflows.

By choosing Terrific Broth from IBI Scientific, you’re investing in a proven medium that delivers exceptional results for all your E. coli cultivation needs. Order now to experience the difference in plasmid production, protein expression, and bacterial growth efficiency!